Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model.

ABSTRACT: Although the physiological function of the omentum remains elusive, it has been proposed that it plays an important role in fat storage, immune regulation, and regeneration of injured tissues and organs. Although the omentum undergoes expansion upon activation, reports on the accurate assessment of increased cell types and the origin of the increased cells remain limited. To investigate this aspect, the omenta of parabiotic mice were subjected to activation using distinct fluorescent markers and single-cell RNA sequencing (scRNA-seq) was performed to identify circulation-derived omental cells. We found that a considerable number of circulating cells contributed to the activation of the omentum. The omental cells derived from circulating cells exhibited morphological features similar to tho

SUBMITTER: Ishigaki K 

PROVIDER: S-EPMC8260800 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature