Mettl14-Mediated m⁶A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.

ABSTRACT:

Background & aims

N⁶-methyladenosine (m⁶A), the most prevalent and dynamic posttranscriptional methylation modification of mammalian mRNA, is involved in various biological processes, but its role in liver regeneration has not been characterized.

Methods

We first conducted transcriptome-wide m⁶A mRNA sequencing and characterized the expression pattern of m⁶A in regenerating mouse liver. Next, we generated hepatocyte-specific Mettl3- or Mettl14-deficient mice and investigated their role in liver regeneration. A series of biochemical experiments in vitro and in vivo was further performed to investigate potential mechanisms.

Results

We identified an overwhelming proportion of m⁶A-modified genes with initially up-regu

SUBMITTER: Cao X 

PROVIDER: S-EPMC8261664 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature