The effects of genotype on inflammatory response in hippocampal progenitor cells: A computational approach.

ABSTRACT: Cell culture models are valuable tools to study biological mechanisms underlying health and disease in a controlled environment. Although their genotype influences their phenotype, subtle genetic variations in cell lines are rarely characterised and taken into account for in vitro studies. To investigate how the genetic makeup of a cell line might affect the cellular response to inflammation, we characterised the single nucleotide variants (SNPs) relevant to inflammation-related genes in an established hippocampal progenitor cell line (HPC0A07/03C) that is frequently used as an in vitro model for hippocampal neurogenesis (HN). SNPs were identified using a genotyping array, and genes associated with chronic inflammatory and neuroinflammatory response gene ontology terms were retrieved using

SUBMITTER: Lee H 

PROVIDER: S-EPMC8261829 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature