Identification of novel genes involved in apoptosis of HIV-infected macrophages using unbiased genome-wide screening.

ABSTRACT:

Background

Macrophages, besides resting latently infected CD4+ T cells, constitute the predominant stable, major non-T cell HIV reservoirs. Therefore, it is essential to eliminate both latently infected CD4+ T cells and tissue macrophages to completely eradicate HIV in patients. Until now, most of the research focus is directed towards eliminating latently infected CD4+ T cells. However, few approaches have been directed at killing of HIV-infected macrophages either in vitro or in vivo. HIV infection dysregulates the expression of many host genes essential for the survival of infected cells. We postulated that exploiting this alteration may yield novel targets for the selective killing of infected macrophages.

Methods

We applied a pooled shRNA-based genome-wide approach by e

SUBMITTER: Dong SXM 

PROVIDER: S-EPMC8261936 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature