Optimization of the feeding rate of Anopheles farauti s.s. colony mosquitoes in direct membrane feeding assays.

ABSTRACT:

Background

Direct membrane feeding assays (DMFA) are an important tool to study parasite transmission to mosquitoes. Mosquito feeding rates in these artificial systems require optimization, as there are a number of factors that potentially influence the feeding rates and there are no standardized methods that apply to all anopheline species.

Methods

A range of parameters prior to and during direct membrane feeding (DMF) were evaluated for their impact on Anopheles farauti sensu stricto feeding rates, including the starving conditions and duration of starving prior to feeding, membrane type, DMF exposure time, mosquito age, feeding in the light versus the dark, blood volume, mosquito density and temperature of water bath.

Results

The average successful DMFA feeding rat

SUBMITTER: Timinao L 

PROVIDER: S-EPMC8261992 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature