Sequential Photoactivation of Self-Assembled Monolayers to Direct Cell Adhesion and Migration.

ABSTRACT: Dynamic substrates for cell culture control the spatial and temporal presentation of extracellular matrix ligands that interact with adherent cells. This paper reports a photoactive surface chemistry that can repeatedly activate regions of the substrate for cell adhesion, spreading, and migration. The approach uses self-assembled monolayers presenting the integrin ligand RGD that is caged with a nitrophenyl-based photoprotecting group. The group is also modified with a maltoheptaose oligosaccharide to prevent nonspecific protein adsorption and cell attachment. The peptide is uncaged when irradiated with a laser source at 405 nm on a microscope to reveal micron-size regions for single cell attachment. This method is applied to studies of gap junction-mediated communication between two neigh

SUBMITTER: Bugga P 

PROVIDER: S-EPMC8262134 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature