A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2.

ABSTRACT: Studies have demonstrated that SARS-CoV-2 RNA can be detected in the feces of infected individuals. This finding spurred investigation into using wastewater-based epidemiology (WBE) to monitor SARS-CoV-2 RNA and track the appearance and spread of COVID-19 in communities. SARS-CoV-2 is present at low levels in wastewater, making sample concentration a prerequisite for sensitive detection and utility in WBE. Whereas common methods for isolating viral genetic material are biased toward intact virus isolation, it is likely that a relatively low percentage of the total SARS-CoV-2 RNA genome in wastewater is contained within intact virions. Therefore, we hypothesized that a direct unbiased total nucleic acid(TNA) extraction method could overcome the cumbersome protocols, variability and low reco

SUBMITTER: Mondal S 

PROVIDER: S-EPMC8262391 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature