Mapping and validating stem rust resistance genes directly in self-incompatible genetic resources of winter rye.

ABSTRACT:

Key message

Individual stem rust resistance genes could be directly mapped within self-incompatible rye populations. Genetic resources of rye (Secale cereale L.) are cross-pollinating populations that can be highly diverse and are naturally segregating. In this study, we show that this segregation could be used for mapping stem rust resistance. Populations of pre-selected donors from the Russian Federation, the USA and Austria were tested on a single-plant basis for stem rust resistance by a leaf-segment test with three rust isolates. Seventy-four plants per population were genotyped with a 10 K-SNP chip. Using cumulative logit models, significant associations between the ordinal infection score and the marker alleles could be found. Three different loci (Pgs1, Pgs2, Pgs3) in three

SUBMITTER: Gruner P 

PROVIDER: S-EPMC8263455 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature