A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation.

ABSTRACT: The development of livers-on-a-chip aims to provide pharmaceutical companies with reliable systems to perform drug screening and toxicological studies. To that end, microfluidic systems are engineered to mimic the functions and architecture of this organ. In this context we have designed a device that reproduces series of liver microarchitectures, each permitting the 3D culture of hepatocytes by confining them to a chamber that is separated from the medium conveying channel by very thin slits. We modified the structure to ensure its compatibility with the culture of hepatocytes from different sources. Our device was adapted to the migratory and adhesion properties of the human HepaRG cell line at various stages of differentiation. Using this device, it was possible to keep the cells alive

SUBMITTER: Boul M 

PROVIDER: S-EPMC8263583 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature