M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity.

ABSTRACT: Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcripti

SUBMITTER: Bag I 

PROVIDER: S-EPMC8263732 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature