Cost-efficient and user-friendly 17O/18O labeling procedures of fatty acids using mechanochemistry.
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ABSTRACT:
SUBMITTER: Spackova J
PROVIDER: S-EPMC8265319 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Cost-efficient and user-friendly <sup>17</sup>O/<sup>18</sup>O labeling procedures of fatty acids using mechanochemistry.
Špačková Jessica J Fabra Charlyn C Cazals Guillaume G Hubert-Roux Marie M Schmitz-Afonso Isabelle I Goldberga Ieva I Berthomieu Dorothée D Lebrun Aurélien A Métro Thomas-Xavier TX Laurencin Danielle D
Chemical communications (Cambridge, England) 20210701 55
Two mechanochemical procedures for 17O/18O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid (DHA). Overall, a total of 9 pure labeled products were isolated in high yields (≥80%) and with high enrichment levels (≥37% average labeling of C=O and C-OH carboxylic oxygen atoms), under mild condition ...[more]
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