Biotransformation of Doxorubicin Promotes Resilience in Simplified Intestinal Microbial Communities.

ABSTRACT: Chemotherapeutic drugs can cause harmful gastrointestinal side effects, which may be modulated by naturally occurring members of our microbiome. We constructed simplified gut-associated microbial communities to test the hypothesis that bacteria-mediated detoxification of doxorubicin (i.e., a widely used chemotherapeutic) confers protective effects on the human microbiota. Mock communities composed of up to five specific members predicted by genomic analysis to be sensitive to the drug or resistant via biotransformation and/or efflux were grown in vitro over three generational stages to characterize community assembly, response to perturbation (doxorubicin exposure), and resilience. Bacterial growth and drug concentrations were monitored with spectrophotometric assays, and strain rel

SUBMITTER: Blaustein RA 

PROVIDER: S-EPMC8265622 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature