BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae.

ABSTRACT: Human bromodomain and extra-terminal domain (BET) family members are promising targets for therapy of cancer and immunoinflammatory diseases, but their mechanisms of action and functional redundancies are poorly understood. Bdf1/2, yeast homologues of the human BET factors, were previously proposed to target transcription factor TFIID to acetylated histone H4, analogous to bromodomains that are present within the largest subunit of metazoan TFIID. We investigated the genome-wide roles of Bdf1/2 and found that their important contributions to transcription extend beyond TFIID function as transcription of many genes is more sensitive to Bdf1/2 than to TFIID depletion. Bdf1/2 co-occupy the majority of yeast promoters and affect preinitiation complex formation through recruitment of TFIID, Med

SUBMITTER: Donczew R 

PROVIDER: S-EPMC8266393 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature