DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies.

ABSTRACT: In Escherichia coli, the DnaB helicase forms the basis for the assembly of the DNA replication complex. The stability of DnaB at the replication fork is likely important for successful replication initiation and progression. Single-molecule experiments have significantly changed the classical model of highly stable replication machines by showing that components exchange with free molecules from the environment. However, due to technical limitations, accurate assessments of DnaB stability in the context of replication are lacking. Using in vitro fluorescence single-molecule imaging, we visualise DnaB loaded on forked DNA templates. That these helicases are highly stable at replication forks, indicated by their observed dwell time of ∼30 min. Addition of the remaining replication factors re

SUBMITTER: Spinks RR 

PROVIDER: S-EPMC8266626 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature