Efficient splicing-based RNA regulators for tetracycline-inducible gene expression in human cell culture and C. elegans.

ABSTRACT: Synthetic riboswitches gain increasing interest for controlling transgene expression in diverse applications ranging from synthetic biology, functional genomics, and pharmaceutical target validation to potential therapeutic approaches. However, existing systems often lack the pharmaceutically suited ligands and dynamic responses needed for advanced applications. Here we present a series of synthetic riboswitches for controlling gene expression through the regulation of alternative splicing. Placing the 5'-splice site into a stem structure of a tetracycline-sensing aptamer allows us to regulate the accessibility of the splice site. In the presence of tetracycline, an exon with a premature termination codon is skipped and gene expression can occur, whereas in its absence the exon is included

SUBMITTER: Finke M 

PROVIDER: S-EPMC8266659 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature