Chromatin-directed proteomics-identified network of endogenous androgen receptor in prostate cancer cells.

ABSTRACT: Treatment of prostate cancer confronts resistance to androgen receptor (AR)-targeted therapies. AR-associated coregulators and chromatin proteins hold a great potential for novel therapy targets. Here, we employed a powerful chromatin-directed proteomics approach termed ChIP-SICAP to uncover the composition of chromatin protein network, the chromatome, around endogenous AR in castration resistant prostate cancer (CRPC) cells. In addition to several expected AR coregulators, the chromatome contained many nuclear proteins not previously associated with the AR. In the context of androgen signaling in CRPC cells, we further investigated the role of a known AR-associated protein, a chromatin remodeler SMARCA4 and that of SIM2, a transcription factor without a previous association with AR. To un

SUBMITTER: Launonen KM 

PROVIDER: S-EPMC8266679 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature