Overexpression of a single ORF can extend chronological lifespan in yeast if retrograde signaling and stress response are stimulated.

ABSTRACT: Systematic collections of single-gene deletions have been invaluable in uncovering determinants of lifespan in yeast. Overexpression of a single gene does not have such a clear outcome as cancellation of its function but it can lead to a variety of imbalances, deregulations and compensations, and some of them could be important for longevity. We report an experiment in which a genome-wide collection of strains overexpressing a single gene was assayed for chronological lifespan (CLS). Only one group of proteins, those locating to the inner membrane and matrix of mitochondria, tended to extend CLS when abundantly overproduced. We selected two such strains-one overexpressing Qcr7 of the respiratory complex III, the other overexpressing Mrps28 of the small mitoribosomal subunit-and analyzed th

SUBMITTER: Pogoda E 

PROVIDER: S-EPMC8266792 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature