Dysregulation of bisphosphoglycerate mutase during in vitro maturation of oocytes.

ABSTRACT:

Purpose

Oxygen is vital for oocyte maturation; however, oxygen regulation within ovarian follicles is not fully understood. Hemoglobin is abundant within the in vivo matured oocyte, indicating potential function as an oxygen regulator. However, hemoglobin is significantly reduced following in vitro maturation (IVM). The molecule 2,3-bisphosphoglycerate (2,3-BPG) is essential in red blood cells, facilitating release of oxygen from hemoglobin. Towards understanding the role of 2,3-BPG in the oocyte, we characterized gene expression and protein abundance of bisphosphoglycerate mutase (Bpgm), which synthesizes 2,3-BPG, and whether this is altered under low oxygen or hemoglobin addition during IVM.

Methods

Hemoglobin and Bpgm expression within in vivo matured human cumulus cells

SUBMITTER: Lim M 

PROVIDER: S-EPMC8266955 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature