Efficacy and safety of papaverine as an in vitro motility enhancer on human spermatozoa.

ABSTRACT:

Purpose

The aim of this study was to examine the ability and safety of papaverine supplementation for in vitro sperm motility enhancement. In addition, sperm motility enhancement of papaverine was compared to pentoxifylline and theophylline. The post-thaw spermatozoa were used as an asthenozoospermia model.

Methods

Post thaw sperm suspensions were divided into two groups: papaverine (100 μmol/L) and control, and each was investigated in two subgroups of 30- and 60-min exposure times. Detailed motility parameters were detected using a computerized sperm motility analyzer. Acrosomal status, viability, apoptosis, and DNA fragmentation were evaluated by flow cytometry. Furthermore, the motility-enhancing capacity of papaverine, pentoxifylline, and theophylline was compared.

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SUBMITTER: Ibis E 

PROVIDER: S-EPMC8266967 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature