DNA repair in primordial follicle oocytes following cisplatin treatment.

ABSTRACT:

Purpose

Genotoxic chemotherapy and radiotherapy can cause DNA double stranded breaks (DSBs) in primordial follicle (PMF) oocytes, which then undergo apoptosis. The development of effective new fertility preservation agents has been hampered, in part, by a limited understanding of DNA repair in PMF oocytes. This study investigated the induction of classical DSB repair pathways in the follicles of wild type (WT) and apoptosis-deficient Puma^-/- mice in response to DSBs caused by the chemotherapy agent cisplatin.

Methods

Adult C57BL/6 WT and Puma^-/- mice were injected i.p. with saline or cisplatin (5 mg/kg); ovaries were harvested at 8 or 24 h. Follicles were counted, and H2A histone family member (γH2AX) immunofluorescence used to demonstrate DSBs. DNA rep

SUBMITTER: Nguyen QN 

PROVIDER: S-EPMC8266985 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature