Optical Coherence Tomography Angiography in the Thirteen-Lined Ground Squirrel.

ABSTRACT:

Purpose

To assess the performance of two spectral-domain optical coherence tomography-angiography systems in a natural model of hypoperfusion: the hibernating thirteen-lined ground squirrel (13-LGS).

Methods

Using a high-speed (130 kHz) OCT-A system (HS-OCT-A) and a commercial OCT (36 kHz; Bioptigen Envisu; BE-OCT-A), we imaged the 13-LGS retina throughout its hibernation cycle. Custom software was used to extract the superior, middle, and deep capillary plexus (SCP, MCP, and DCP, respectively). The retinal vasculature was also imaged with adaptive optics scanning light ophthalmoscopy (AOSLO) during torpor to visualize individual blood cells. Finally, correlative histology with immunolabeled or DiI-stained vasculature was performed.

Results

During euthermia, vessel de

SUBMITTER: Salmon AE 

PROVIDER: S-EPMC8267221 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature