Cellular Diversity and Differential Subcellular Localization of the G-Protein G_αo Subunit in the Mouse Cerebellum.

ABSTRACT: Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce signals from G protein-coupled receptors (GPCRs) to effector ion channels and enzymes G_αo, a member of the pertussis toxin-sensitive G _i/o family, is widely expressed in the brain, although its role within a neuronal context remains largely unknown. Using immunohistochemical and quantitative immunoelectron microscopy techniques, we have investigated the expression, cellular and subcellular localization of G_αo in the cerebellar cortex. Histoblot revealed that G_αo is expressed in many brain regions, including the cerebellum. At the cellular level, G_αo protein was distributed in Purkinje cells, basket cells, stellate cells, granule cells and Golgi cells. At t

SUBMITTER: Roldan-Sastre A 

PROVIDER: S-EPMC8267243 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature