Using Cell Type-Specific Genes to Identify Cell-Type Transitions Between Different in vitro Culture Conditions.

ABSTRACT: In vitro differentiation or expansion of stem and progenitor cells under chemical stimulation or genetic manipulation is used for understanding the molecular mechanisms of cell differentiation and self-renewal. However, concerns around the cell identity of in vitro-cultured cells exist. Bioinformatics methods, which rely heavily on signatures of cell types, have been developed to estimate cell types in bulk samples. The Tabula Muris Senis project provides an important basis for the comprehensive identification of signatures for different cell types. Here, we identified 46 cell type-specific (CTS) gene clusters for 83 mouse cell types. We conducted Gene Ontology term enrichment analysis on the gene clusters and revealed the specific functions of the relevant cell types. Next,

SUBMITTER: He X 

PROVIDER: S-EPMC8267371 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature