Ovarian cancer modulates the immunosuppressive function of CD11b⁺Gr1⁺ myeloid cells via glutamine metabolism.

ABSTRACT:

Objective

Immature CD11b ⁺ Gr1⁺ myeloid cells that acquire immunosuppressive capability, also known as myeloid-derived suppressor cells (MDSCs), are a heterogeneous population of cells that regulate immune responses. Our study's objective was to elucidate the role of ovarian cancer microenvironment in regulating the immunosuppressive function of CD11b⁺Gr1⁺ myeloid cells.

Methods

All studies were performed using the intraperitoneal ID8 syngeneic epithelial ovarian cancer mouse model. Myeloid cell depletion and immunotherapy were carried out using anti-Gr1 mAb, gemcitabine treatments, and/or anti-PD1 mAb. The treatment effect was assessed by a survival curve, in situ luciferase-guided imaging, and histopathologic evaluation. Adoptive

SUBMITTER: Udumula MP 

PROVIDER: S-EPMC8267600 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature