Reversible Lectin Binding to Glycan-Functionalized Graphene.

ABSTRACT: The monolayer character of two-dimensional materials predestines them for application as active layers of sensors. However, their inherent high sensitivity is always accompanied by a low selectivity. Chemical functionalization of two-dimensional materials has emerged as a promising way to overcome the selectivity issues. Here, we demonstrate efficient graphene functionalization with carbohydrate ligands-chitooligomers, which bind proteins of the lectin family with high selectivity. Successful grafting of a chitooligomer library was thoroughly characterized, and glycan binding to wheat germ agglutinin was studied by a series of methods. The results demonstrate that the protein quaternary structure remains intact after binding to the functionalized graphene, and that the lectin can be libera

SUBMITTER: Koukalova T 

PROVIDER: S-EPMC8267698 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature