Characterization of Supragingival Plaque and Oral Swab Microbiomes in Children With Severe Early Childhood Caries.

ABSTRACT: The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) < 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal mic

SUBMITTER: de Jesus VC 

PROVIDER: S-EPMC8267818 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature