Metabolomic Reprogramming of C57BL/6-Macrophages during Early Infection with L. amazonensis.

ABSTRACT: Leishmania survival inside macrophages depends on factors that lead to the immune response evasion during the infection. In this context, the metabolic scenario of the host cell-parasite relationship can be crucial to understanding how this parasite can survive inside host cells due to the host's metabolic pathways reprogramming. In this work, we aimed to analyze metabolic networks of bone marrow-derived macrophages from C57BL/6 mice infected with Leishmania amazonensis wild type (La-WT) or arginase knocked out (La-arg-), using the untargeted Capillary Electrophoresis-Mass Spectrometry (CE-MS) approach to assess metabolomic profile. Macrophages showed specific changes in metabolite abundance upon Leishmania infection, as well as in the absence of parasite-arginase. The absence of L. amazon

SUBMITTER: Mamani-Huanca M 

PROVIDER: S-EPMC8267886 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature