[Expression of nicotinamide-N-methyltransferase in gastric cancer and its biological and clinicopathological significance].

ABSTRACT:

Objective

To investigate the expression of nicotinamide-N-methyltransferase (NNMT) in gastric cancer (GC) and explore its biological and clinicopathological significance.

Objective

We screened the candidate genes associated with the classification and prognosis of gastric cancer by analyzing GEO, Oncomine and TCGA datasets. The molecular pathways and protein interaction network involving these candidate genes were analyzed using STRING, GSEA, David and Cytoscape software. The expressions of the candidate genes in 28 pairs of gastric cancer and adjacent tissues were detected with qRTPCR, and CCK-8 assay, clone formation assay, wound healing assay and Transwell assay were carried out to analyze the effects of modulation of NNMT expression on proliferation, invasion and migrati

SUBMITTER: Wen Z 

PROVIDER: S-EPMC8267982 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature