[miR-483-5p aggravates cisplatin-induced premature ovarian insufficiency in rats by targeting FKBP4].

ABSTRACT:

Objective

To investigate the role of FKBP4 protein in cisplatin-induced premature ovarian insufficiency (POI).

Objective

We performed ITRAQ assay of the ovarian tissues from 4 mice with cisplatin-induced POI and 4 control mice, and identified FKBP4 as a significantly down-regulated protein in the oocytes and granulosa cells following cisplatin treatment. TargetScan software was used for target analysis of FKBP4, and qRT-PCR and Western blotting were used to verify the expression levels of miR-483-5p and FKBP4 in the mouse models. Serum samples were collected from patients with POI and healthy women for detecting miR-483-5p level with qRT-PCR. Cell transfection and dual-luciferase assay were performed to determine the relationship between miR-483-5p and FKBP4. In primary gran

SUBMITTER: Zhao H 

PROVIDER: S-EPMC8267993 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature