Loureirin B Exerts its Immunosuppressive Effects by Inhibiting STIM1/Orai1 and K_V1.3 Channels.

ABSTRACT: Loureirin B (LrB) is a constituent extracted from traditional Chinese medicine Resina Draconis. It has broad biological functions and an impressive immunosuppressive effect that has been supported by numerous studies. However, the molecular mechanisms underlying Loureirin B-induced immune suppression are not fully understood. We previously reported that Loureirin B inhibited K_V1.3 channel, calcium ion (Ca²⁺) influx, and interleukin-2 (IL-2) secretion in Jurkat T cells. In this study, we applied CRISPR/Cas9 to edit K_V1.3 coding gene KCNA3 and successfully generated a K_V1.3 knockout (KO) cell model to determine whether K_V1.3 KO was sufficient to block the Loureirin B-induced immunosuppressive effect. Surprisingly, we showed that Loureiri

SUBMITTER: Shi S 

PROVIDER: S-EPMC8268022 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature