Unknown

Dataset Information

0

C-Terminal Fragment of Vitellogenin II, a Potential Yolkin Polypeptide Complex Precursor Protein-Heterologous Expression, Purification, and Immunoregulatory Activity.

ABSTRACT: The aim of this research was to analyze the heterologous expression, purification, and immunoregulatory activity of recombinant YGP40 (rYGP40), the potential precursor of the yolkin peptide complex. The ygp40 coding sequence was codon optimized, successfully expressed in the E. coli system, and purified from inclusion bodies with a yield of about 1.1 mg/L of culture. This study showed that the protein exhibits immunomodulatory activity, expressed by the stimulation of TNF-α and IL-10 production and nitric oxide induction at a level comparable to that of the natural yolkin peptide complex obtained by other authors from hen egg yolk. At the highest dose of 100 µg/mL, rYGP40 also caused the up-regulation of iNOS expression in murine bone marrow-derived macrophages (BMDM). Moreover, no cytotoxic effects of rYGP40 on the BMDM cell line were observed.

SUBMITTER: Szmyt A 

PROVIDER: S-EPMC8268165 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

<i>C</i>-Terminal Fragment of Vitellogenin II, a Potential Yolkin Polypeptide Complex Precursor Protein-Heterologous Expression, Purification, and Immunoregulatory Activity.

Szmyt Agnieszka A   Zabłocka Agnieszka A   Macała Józefa J   Chrzanowska Józefa J   Dąbrowska Anna A  

International journal of molecular sciences 20210705 13

The aim of this research was to analyze the heterologous expression, purification, and immunoregulatory activity of recombinant YGP40 (rYGP40), the potential precursor of the yolkin peptide complex. The ygp40 coding sequence was codon optimized, successfully expressed in the <i>E. coli</i> system, and purified from inclusion bodies with a yield of about 1.1 mg/L of culture. This study showed that the protein exhibits immunomodulatory activity, expressed by the stimulation of TNF-α and IL-10 prod  ...[more]

Similar Datasets

Unknown High expression and purification of amino-terminal fragment of human amyloid precursor protein in Pichia pastoris and partial analysis of its properties.
| S-EPMC3838808 | biostudies-literature
Unknown Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins.
| S-EPMC9373356 | biostudies-literature
Unknown Dopamine determines the vulnerability of striatal neurons to the N-terminal fragment of mutant huntingtin through the regulation of mitochondrial complex II.
| S-EPMC2367694 | biostudies-literature
Unknown Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus.
| S-EPMC3027471 | biostudies-literature
Unknown [Prokaryotic expression and purification of human TLE1 N-terminal Q domain fragment and production of its polyclonal antibody].
| S-EPMC6000491 | biostudies-literature
Unknown Purification and characterization of brochocin A and brochocin B(10-43), a functional fragment generated by heterologous expression in Carnobacterium piscicola.
| S-EPMC150086 | biostudies-literature
Unknown Crystal structure of yeast Sis1 peptide-binding fragment and Hsp70 Ssa1 C-terminal complex.
| S-EPMC1559466 | biostudies-literature
Unknown TMEM30A is a candidate interacting partner for the β-carboxyl-terminal fragment of amyloid-β precursor protein in endosomes.
| S-EPMC6080755 | biostudies-literature
Unknown Heterologous expression, purification, crystallization and preliminary X-ray analysis of Trichoderma reesei xylanase II and four variants.
| S-EPMC3606583 | biostudies-literature
Unknown Chimeric Virus-like Particles Co-Displaying Hemagglutinin Stem and the C-Terminal Fragment of DnaK Confer Heterologous Influenza Protection in Mice.
| S-EPMC9610613 | biostudies-literature