MiR-125b inhibits cardiomyocyte apoptosis by targeting BAK1 in heart failure.

ABSTRACT:

Background

Although miR-125b plays a crucial role in many human cancers. However, its function in heart failure (HF) remains unclear. Our study aimed to investigate its involvement in heart failure.

Methods

In this study, the mouse HF model was successfully constructed through transverse aortic constriction (TAC) operation. Changes in mRNA and protein levels in isolated myocytes and heart tissues were examined using qRT-PCR, Western blot and Immunohistochemical staining and immunofluorescent staining. Changes in cardiac functions were examined using ultrasound. Interactions between miR-125b and BAK1 was analyzed using the luciferase reporter assay. Cardiomyocyte apoptosis was evaluated using the TUNEL staining.

Results

We found that miR-125b expression was significant

SUBMITTER: Zhang B 

PROVIDER: S-EPMC8268255 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature