Effect of Endothelial Culture Medium Composition on Platelet Responses to Polymeric Biomaterials.

ABSTRACT: Near-physiological in vitro thrombogenicity test systems for the evaluation of blood-contacting endothelialized biomaterials requires co-cultivation with platelets (PLT). However, the addition of PLT has led to unphysiological endothelial cell (EC) detachment in such in vitro systems. A possible cause for this phenomenon may be PLT activation triggered by the applied endothelial cell medium, which typically consists of basal medium (BM) and nine different supplements. To verify this hypothesis, the influence of BM and its supplements was systematically analyzed regarding PLT responses. For this, human platelet rich plasma (PRP) was mixed with BM, BM containing one of nine supplements, or with BM containing all supplements together. PLT adherence analysis was carried out in six-channel slid

SUBMITTER: Lau S 

PROVIDER: S-EPMC8268423 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature