Characterization of the effects of heat stress on autophagy induction in the pig oocyte.

ABSTRACT:

Background

Heat stress (HS) occurs when body heat accumulation exceeds heat dissipation and is associated with swine seasonal infertility. HS contributes to compromised oocyte integrity and reduced embryo development. Autophagy is a potential mechanism for the oocyte to mitigate the detrimental effects of HS by recycling damaged cellular components.

Methods

To characterize the effect of HS on autophagy in oocyte maturation, we utilized an in vitro maturation (IVM) system where oocytes underwent thermal neutral (TN) conditions throughout the entire maturation period (TN/TN), HS conditions during the first half of IVM (HS/TN), or HS conditions during the second half of IVM (TN/HS).

Results

To determine the effect of HS on autophagy induction within the oocyte, we compar

SUBMITTER: Hale BJ 

PROVIDER: S-EPMC8268447 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature