Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii.

ABSTRACT: The spotted-wing Drosophila (Drosophila suzukii Matsumura) is native to eastern Asia, but has become a global threat to fruit production. In recent years, CRISPR/Cas9 targeting was established in this species allowing for functional genomic and genetic control studies. Here, we report the generation and characterization of Cas9-expressing strains of D. suzukii. Five independent transgenic lines were generated using a piggyBac construct containing the EGFP fluorescent marker gene and the Cas9 gene under the control of the D. melanogaster heat shock protein 70 promoter and 3'UTR. Heat-shock (HS) treated embryos were analyzed by reverse transcriptase PCR, revealing strong heat inducibility of the transgenic Cas9 expression. By injecting gRNA ta

SUBMITTER: Yan Y 

PROVIDER: S-EPMC8268499 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature