Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents.

ABSTRACT: Human plasma-derived bilayered skin substitutes were successfully used by our group to produce human-based in vitro skin models for toxicity, cosmetic, and pharmaceutical testing. However, mechanical weakness, which causes the plasma-derived fibrin matrices to contract significantly, led us to attempt to improve their stability. In this work, we studied whether an increase in fibrin concentration from 1.2 to 2.4 mg/mL (which is the useful fibrinogen concentration range that can be obtained from plasma) improves the matrix and, hence, the performance of the in vitro skin cultures. The results show that this increase in fibrin concentration indeed affected the mechanical properties by doubling the elastic moduli and the maximum load. A structural analysis indicated a decreased porosity for t

SUBMITTER: Montero A 

PROVIDER: S-EPMC8269027 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature