MRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease.

ABSTRACT:

Aim

we aimed to construct a bioinformatics-based co-regulatory network of mRNAs and non coding RNAs (ncRNAs), which is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), followed by its validation in a NAFLD animal model.

Materials and methods

The mRNAs-miRNAs-lncRNAs regulatory network involved in NAFLD was retrieved and constructed utilizing bioinformatics tools. Then, we validated this network using an NAFLD animal model, high sucrose and high fat diet (HSHF)-fed rats. Finally, the expression level of the network players was assessed in the liver tissues using reverse transcriptase real-time polymerase chain reaction.

Results

in-silico constructed network revealed six mRNAs (YAP1, FOXA2, AMOTL2, TEAD2, SMAD4 and NF2), two miR

SUBMITTER: Matboli M 

PROVIDER: S-EPMC8269036 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature