MetFish: a Metabolomics Pipeline for Studying Microbial Communities in Chemically Extreme Environments.

ABSTRACT: Metabolites have essential roles in microbial communities, including as mediators of nutrient and energy exchange, cell-to-cell communication, and antibiosis. However, detecting and quantifying metabolites and other chemicals in samples having extremes in salt or mineral content using liquid chromatography-mass spectrometry (LC-MS)-based methods remains a significant challenge. Here, we report a facile method based on in situ chemical derivatization followed by extraction for analysis of metabolites and other chemicals in hypersaline samples, enabling for the first time direct LC-MS-based exometabolomics analysis in sample matrices containing up to 2 M total dissolved salts. The method, MetFish, is applicable to molecules containing amine, carboxylic acid, carbonyl, or hydroxyl func

SUBMITTER: Xu C 

PROVIDER: S-EPMC8269250 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature