NLRP3 inflammasome activation mediates sleep deprivation-induced pyroptosis in mice.

ABSTRACT:

Background

Sleep deprivation (SD) has many deleterious health effects, including cognitive decline, work ability decline, inadequate alertness, etc. Neuroinflammation plays an important role in sleep deprivation. However, the underlying mechanism is still unclear.

Methods

In the present study, we detected the activation of microglia and apoptosis of nerve cells in sleep deprivation (SD) mice model using IHC, HE staining and TUNEL apoptosis assay. RT-PCR array data were used to detect the expression of inflammatory bodies in hippocampal CA1 region after sleep deprivation, to explore how NLRP3 inflammasome regulates neuronal apoptosis and how specific signaling pathways are involved in SD-induced activation of NLRP3/pyrosis axis.

Results

We found the number of microglia

SUBMITTER: Fan K 

PROVIDER: S-EPMC8269641 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature