Development and analytical validation of a novel bioavailable 25-hydroxyvitamin D assay.

ABSTRACT:

Background

Bioavailable 25-hydroxyvitamin D (25OHD) may be a better indicator of vitamin D sufficiency than total 25OHD. This report describes a novel assay for measuring serum bioavailable 25OHD.

Methods

We developed an assay for 25OHD % bioavailability based on competitive binding of 25OHD tracer between vitamin D-binding protein (DBP)-coated affinity chromatography beads and serum DBP. Bioavailable 25OHD, total 25OHD, albumin, and DBP protein concentrations were measured in 89 samples from hospitalized patients and 42 healthy controls to determine how the DBP binding assay responds to differences in concentrations of DBP and compares to calculated bioavailable 25OHD values.

Results

DBP binding assay showed a linear relationship between DBP-bound 25OHD tracer recove

SUBMITTER: Berg AH 

PROVIDER: S-EPMC8270209 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature