Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition.

ABSTRACT: AMPA-selective glutamate receptors mediate the transduction of signals between the neuronal circuits of the hippocampus¹. The trafficking, localization, kinetics and pharmacology of AMPA receptors are tuned by an ensemble of auxiliary protein subunits, which are integral membrane proteins that associate with the receptor to yield bona fide receptor signalling complexes². Thus far, extensive studies of recombinant AMPA receptor-auxiliary subunit complexes using engineered protein constructs have not been able to faithfully elucidate the molecular architecture of hippocampal AMPA receptor complexes. Here we obtain mouse hippocampal, calcium-impermeable AMPA receptor complexes using immunoaffinity purification and use single-molecule fluorescence and cryo-electron micros

SUBMITTER: Yu J 

PROVIDER: S-EPMC8270219 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature