Aquaporin 4 differentially modulates osmotic effects on vasopressin neurons in rat supraoptic nucleus.

ABSTRACT:

Aim

Glial fibrillary acidic protein (GFAP) molecularly associates with aquaporin 4 (AQP4) in astrocytic plasticity. Here, we further examined how AQP4 modulates osmotic effects on vasopressin (VP) neurons in rat supraoptic nucleus (SON) through interactions with GFAP in astrocytes.

Methods

Brain slices from adult male rats were kept under osmotic stimulation. Western blot, co-immunoprecipitation, immunohistochemistry and patch-clamp recordings were used for analysis of expressions and interactions between GFAP and AQP4, astrocyte-specific proteins in the SON, as well as their influence on VP neuronal activity. Data were analysed using SPSS software.

Results

Hyposmotic challenge (HOC) of acute SON slices caused an early (within 5 minutes) and transient increase in the

SUBMITTER: Wang X 

PROVIDER: S-EPMC8270393 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature