Multinuclei Occurred Under Cryopreservation and Enhanced the Pathogenicity of Melampsora larici-populina.

ABSTRACT: Melampsora larici-populina is a macrocyclic rust, and the haploid stage with two nuclei and the diploid of mononuclear sequentially occur annually. During the preservation of dry urediniospores at -80°C, we found that one isolate, ΔTs₀₆, was different from the usual wild-type isolate Ts₀₆ at -20°C because it has mixed polykaryotic urediniospores. However, the other spores, including the 0, I, III, and IV stages of a life cycle, were the same as Ts₀₆. After five generations of successive inoculation and harvest of urediniospores from the compatible host Populus purdomii, the isolate ΔTs₀₆ steadily maintained more than 20% multiple nucleus spores. To test the pathogenesis variation of ΔTs₀₆, an assay of host poplars was app

SUBMITTER: Zheng W 

PROVIDER: S-EPMC8270653 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature