Pairs of amino acids at the P- and A-sites of the ribosome predictably and causally modulate translation-elongation rates.

ABSTRACT: Variation in translation-elongation kinetics along a transcript's coding sequence plays an important role in the maintenance of cellular protein homeostasis by regulating co-translational protein folding, localization, and maturation. Translation-elongation speed is influenced by molecular factors within mRNA and protein sequences. For example, the presence of proline in the ribosome's P- or A-site slows down translation, but the effect of other pairs of amino acids, in the context of all 400 possible pairs, has not been characterized. Here, we study Saccharomyces cerevisiae using a combination of bioinformatics, mutational experiments, and evolutionary analyses, and show that many different pairs of amino acids and their associated tRNA molecules predictably and causally encode translatio

SUBMITTER: Ahmed N 

PROVIDER: S-EPMC8270670 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature