Complement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice.

ABSTRACT: Complement impacts innate and adaptive immunity. Using a model in which the human KEL glycoprotein is expressed on murine red blood cells (RBCs), we have shown that polyclonal immunoprophylaxis (KELIg) prevents alloimmunization to transfused RBCs when a recipient is in their baseline state of heath but with immunoprophylaxis failure occurring in the presence of a viral-like stimulus. As complement can be detected on antibody coated KEL RBCs following transfusion, we hypothesized that recipient complement synergizes with viral-like inflammation to reduce immunoprophylaxis efficacy. Indeed, we found recipient C3 and C1q were critical to immunoprophylaxis failure in the setting of a viral-like stimulus, with no anti-KEL IgG alloantibodies generated in C3^-/- or C1q^-/- mic

SUBMITTER: Escamilla-Rivera V 

PROVIDER: S-EPMC8270673 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature