Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells.

ABSTRACT:

Objective

To investigate the role of PI3K/AKT signaling pathway in nucleus pulposus (NP) cells.

Methods

Nucleus pulposus (NP) cells were isolated from SD rat, and thereafter, passage three (P3) NP cells were divided into the following experimental groups: control, PI3K/AKT agonist IGF-1 (25 ng/ml, 50 ng/ml, and 100 ng/ml), and PI3K/AKT inhibitor LY294002 (5 μM, 10 μM, and 20 μM). Flow cytometry and BrdU cell proliferation assays were performed to assess apoptosis and the proliferation rate of NP cells. Western blot analysis was performed to examine the protein expression level of Col II, Col X, Aggrecan, and MMP13.

Results

PI3K/AKT inhibitor LY294002 increased the rate of apoptosis in NP cells when compared to the control and decreased the prolife

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC8270693 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature