Stratigraphy of stable isotope ratios and leaf structure within an African rainforest canopy with implications for primate isotope ecology.

ABSTRACT: The canopy effect describes vertical variation in the isotope ratios of carbon (δ¹³C), oxygen (δ¹⁸O) and partially nitrogen (δ¹⁵N) within plants throughout a closed canopy forest, and may facilitate the study of canopy feeding niches in arboreal primates. However, the nuanced relationship between leaf height, sunlight exposure and the resulting variation in isotope ratios and leaf mass per area (LMA) has not been documented for an African rainforest. Here, we present δ¹³C, δ¹⁸O and δ¹⁵N values of leaves (n = 321) systematically collected from 58 primate food plants throughout the canopy (0.3 to 42 m) in Côte d'Ivoire, West Africa. Besides leaf sample height and light availability, we measured leaf nitrogen and carbon content (%N, %C), as well as LMA (n = 214) to address the plants' vertical resource allocations. We found significant variation in δ¹³C, δ¹⁸O and δ¹⁵N, as well as LMA in response to height in combination with light availability and tree species, with low canopy leaves depleted in ¹³C, ¹⁸O and ¹⁵N and slightly higher in %N compared to higher canopy strata. While this vertical isotopic variation was not well reflected in the δ¹³C and δ¹⁵N of arboreal primates from this forest, it did correspond well to primate δ¹⁸O values.

SUBMITTER: Lowry BE 

PROVIDER: S-EPMC8270916 | biostudies-literature |

REPOSITORIES: biostudies-literature