Time course profiling of host cell response to herpesvirus infection using nanopore and synthetic long-read transcriptome sequencing.

ABSTRACT: Third-generation sequencing is able to read full-length transcripts and thus to efficiently identify RNA molecules and transcript isoforms, including transcript length and splice isoforms. In this study, we report the time-course profiling of the effect of bovine alphaherpesvirus type 1 on the gene expression of bovine epithelial cells using direct cDNA sequencing carried out on MinION device of Oxford Nanopore Technologies. These investigations revealed a substantial up- and down-regulatory effect of the virus on several gene networks of the host cells, including those that are associated with antiviral response, as well as with viral transcription and translation. Additionally, we report a large number of novel bovine transcript isoforms identified by nanopore and synthetic long-read seq

SUBMITTER: Maroti Z 

PROVIDER: S-EPMC8270970 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature