3D MAS NMR Experiment Utilizing Through-Space ¹⁵N-¹⁵N Correlations.

ABSTRACT: We demonstrate a novel 3D NNC magic angle spinning NMR experiment that generates ¹⁵N-¹⁵N internuclear contacts in protein systems using an optimized ¹⁵N-¹⁵N proton assisted recoupling (PAR) mixing period and a ¹³C dimension for improved resolution. The optimized PAR condition permits the acquisition of high signal-to-noise 3D data that enables backbone chemical shift assignments using a strategy that is complementary to current schemes. The spectra can also provide distance constraints. The utility of the experiment is demonstrated on an M₀Aβ_1-42 fibril sample that yields high-quality data that is readily assigned and interpreted. The 3D NNC experiment therefore provides a powerful platform for solid-state protein

SUBMITTER: Donovan KJ 

PROVIDER: S-EPMC8271350 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature