Deciphering the Role of π-Interactions in Polyelectrolyte Complexes Using Rationally Designed Peptides.

ABSTRACT: Electrostatic interactions, and specifically π-interactions play a significant role in the liquid-liquid phase separation of proteins and formation of membraneless organelles/or biological condensates. Sequence patterning of peptides allows creating protein-like structures and controlling the chemistry and interactions of the mimetic molecules. A library of oppositely charged polypeptides was designed and synthesized to investigate the role of π-interactions on phase separation and secondary structures of polyelectrolyte complexes. Phenylalanine was chosen as the π-containing residue and was used together with lysine or glutamic acid in the design of positively or negatively charged sequences. The effect of charge density and also the substitution of fluorine on the phenylalanine ring, kno

SUBMITTER: Tabandeh S 

PROVIDER: S-EPMC8271475 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature